Bleeding canker of horse chestnut - Pseudomonas syringae pv. aesculi
Effective: April 15, 2011 - December 31, 2013
Taxonomic Position: Pseudomonadales : Pseudomonadaceae
Pest Type: Bacteria
Pest Code (NAPIS): FDARPEN
These Approved Methods are appropriate for: 2013, 2012
Pest Information
Pest is vectored by:
Survey

Approved Method(s)
Method Detail NAPIS Survey Method
Visual Collect symptomatic material. 3031 - General Visual Observation

Survey Recommendations
The following are recommendations for executing the survey using the approved methods for pest surveillance. The recommendations are developed through literature review and consultation with subject matter experts.
Signs:
Symptoms:
Key Diagnostic or Identification

Approved Method(s)
ID/Diagnostic:
Mistaken Identities:
In Progress / Literature-based Diagnostics:
References
  1. Bereswill, S., Bugert, P, Volksch, B., Ullrich, M., Bender, C.L., and Geider, K. 1994. Identification and relatedness of coronatine-producing Pseudomonas syringae pathovars by PCR analysis and sequence determination of the amplification products. Applied and Environmental Microbiology 60(8): 2924-2930.
  2. Bultreys, A., Gheysen, I., and Planchon, V. 2008. Characterization of Pseudomonas syringae strains isolated from diseased horse-chestnut trees in Belgium. In: Fatmi et al., (eds.) Pseudomonas syringae pathovars and related pathogens. Springer Science.
  3. Glickman, E., Gardan, L., Jacquet, S., Hussain, S., Elasri, M., Petit, A., and Dessaux, Y. 1998. Auxin production is a common feature of most pathovars of Pseudomonas syringae. Molecular Plant-Microbe Interactions 11(2): 156-162.
  4. Green, S., Laue, B., Fossdal, C.G., A'Hara, S.W., and Cottrell, J.E. 2009. Infection of horse chestnut (Aesculus hippocastanum) by Pseudomonas syringae pv. aesculi and its detection by quantitative real-time PCR. Plant Pathology 58: 731-744.
  5. King, E.O., Ward, M.K., and Raney, D.E. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44: 301-307.
  6. Schmidt, O., Moreth, U., Dujesiefken, D., Stobbe, H., and Gaiser, O. 2009. Fast molecular detection of Pseudomonas syringae pv. aesculi in diseased horse chestnut trees. For. Path. 39: 343-348.
  7. Webber, J., Parkinson, N., Rose, J., Stanford, H., Cook, R.T.A., Elphinistone, J. 2007. Isolation and identification of P. syringae pv. aesculi causing bleeding canker of horse chestnut in the UK. Plant Pathol. New Dis. Rep. 15: 1.
  8. Young, J.M. 2010. Taxonomy of Pseudomonas syringae. Journal of Plant Pathology 91(1): S1.5-S1.14.

If you are unable to find a reference, contact STCAPS@usda.gov. See the CAPS Pest Datasheet for all references.