ID/Diagnostic: Morphological: Keys are available for identification by morphological characteristics of the cyst, second-stage juvenile, male, and female.
Handoo (2002) provides a key to the species within the H. avenae group, as well as a thorough review of morphological studies to date.
Mistaken Identities: H. filipjevi has been confused with several other cyst nematode species that parasitize cereals, including (but not limited to) H. avenae, H. bifenestra, H. hordecalis, H. latipons, H. mani, H. pakistanensis, H. tucomanica, and H. zeae.
Symptoms are often due to mixed populations of cereal cyst nematodes or other soilborne pathogens and can mimic other problems such as nutrient deficiencies or drought stress.
In Progress / Literature-based Diagnostics: For precise identification, other methods are used such as (1) protein electrophoresis, (2) isozymes/ isoelectrofocusing (Andres et al., 2001), and (3) molecular biology techniques, such as polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and sequences of internal transcribed spacer ribosomal DNA (ITS-rDNA) (Bekal et al., 1997; Ferris et al., 1999; Andres et al., 2001; Nicol, 2002; Tanha-Maafi et al., 2003; Rivoal et al., 2003; Subbotin et al., 1999; Subbotin et al., 2001; Subbotin et al., 2003; Madani et al., 2004; Abidou et al., 2005; Yan and Smiley, 2010), and species-specific PCR (Peng et al., 2013; Toumi et al., 2013; Yan et al., 2013).
H. avenae, H. filipjevi, and H. latipons were also differentiated by electrophoresis on cellulose acetate plates using the enzymes esterase and malate dehydrogenase and aliquots of 25 females (Mokabli et al., 2001).