British root-knot nematode - Meloidogyne artiellia
Effective: August 18, 2010
Taxonomic Position: Tylenchida : Heteroderidae
Pest Type: Nematodes
Pest Code (NAPIS): NEOBCBK
This pest is a member of the following surveys: Small Grains
These Approved Methods are appropriate for: 2025, 2024, 2023, 2022, 2021, 2020, 2019, 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011
Pest Information
CAPS Pest Datasheet: https://download.ceris.purdue.edu/file/3129
Pest Tracker: https://pesttracker.org/pest/NEOBCBK
Hosts Identified in the CAPS Host Matrix:
Chickpea; Wheat
Pest is vectored by:
This pest is is not known to be a vector and it is not vectored by any organism.
Survey
Approved Method(s)
| Method | Detail | NAPIS Survey Method |
|---|---|---|
| Soil Sample | Send sample to nematology diagnostic lab where nematodes will be extracted and identified. | 3012 - General Soil Sample |
Survey Recommendations
The following are recommendations for executing the survey using the approved methods for pest surveillance. The recommendations are developed through literature review and consultation with subject matter experts.
Signs:
Root galls induced by M. artiellia are very small and often are covered by large egg masses that represent the only visible signs of the nematode infection.
Symptoms:
Root-knot nematodes (RKNs) symptoms are similar to those caused by nutrient or water deficiency, including stunting, wilting, and chlorosis of leaves. Small root galls and lateral proliferation of roots is also observed with most species.
In wheat, spikes are sparse and reduced in size.
In wheat, spikes are sparse and reduced in size.
Key Diagnostic or Identification
Approved Method(s)
ID/Diagnostic:
1. Assessment of root galls will indicate a possible root knot nematode infection.
2. Morphological: The length of at least 20 second-stage juveniles, the head and stylet morphology of males, and the nature perineal patterns of females from samples.
2. Morphological: The length of at least 20 second-stage juveniles, the head and stylet morphology of males, and the nature perineal patterns of females from samples.
Mistaken Identities:
Meloidogyne artiellia can be confused with other root-knot nematodes, including M. acrita, M. arenaria, M. christiei, M. cruciana, M. megatyla, M. hapla, M. incognita, M. graminis, M. javanica, M. querciana, and M. thamesi.
In Progress / Literature-based Diagnostics:
Molecular: RFLP profiles of the ITS region of rDNA, RAPD fragments, 18SrDNA sequences, satellite DNA probes and species specific primers can distinguish from other species (DiGiorgi et al., 1991; 1994; 2002).
Notes:
Damages cereals, leguminous, and cruciferous crops in Europe and Middle East. This nematode can be introduced with contaminated machinery, soil debris, and rooted propagative plant material.
References
- DiGiorgi, C., De Luca, F., Finetti-Sialer, M., Di Vito, M., and Lamberti, F. 1991. DNA fragments as molecular probes for the diagnosis of plant parasitic nematodes. Nematologia Mediterranea 19: 131-134.
- DiGiorgi, C., Sialer, M.F., Di Vito, M., and Lamberti, F. 1994. Identification of plant-parasitic nematodes by PCR amplification of DNA fragments. EPPO Bulletin 24: 447-451.
- DiGiorgi, C., Veronico, P., De Luca, F., Natilla, A., Lanave, C., and Pesole, G. 2002. Structural and evolutionary analysis of the ribosomal genes of the parasitic nematode Meloidogyne artiellia suggests its ancient origin. Molecular and Biochemical Parasitology 124: 91-94.
If you are unable to find a reference, contact STCAPS@usda.gov. See the CAPS Pest Datasheet for all references.